Fig 2: Cul7 regulates the protein level of Survivin to influence the sensitivity of LUAD cells to docetaxel. (A) Western blotting was performed to measure the protein expression of Cul7 and Survivin in A549, A549DTX, H358 and H358DTX cells. (B) Western blotting was performed to measure the protein level of Survivin in A549DTX-shNeg, A549DTX-shCul7, H358DTX-shNeg, H358DTX-shCul7, A549-pCDNA3, A549-HACul7, H1299-pCDNA3 and H1299-HACul7 cells. (C,D) qPCR was performed to measure the mRNA expression of Survivin in A549DTX-shNeg, A549DTX-shCul7, H358DTX-shNeg, H358DTX-shCul7, A549-pCDNA3, A549-HACul7, H1299-pCDNA3 and H1299-HACul7 cells. (E,F) Survivin expression was acutely knocked down in H1299-HACul7 cells, as verified by qPCR and Western blotting and siRNA233-Survivin small interfering RNA was selected for subsequent experiments. (G) The viability of H1299-HACul7 siRNA-NC and H1299-HACul7 siRNA-Survivin cells treated with various docetaxel concentrations (0, 2, 4, 8, 16, 32, 64, 128, and 256 nM) was evaluated by the MTS method, and the data were analyzed by GraphPad Prism 5 software. (H) The IC50 values obtained via the MTS method in three independent repeated experiments are presented in the form of a histogram. *, P<0.05; **, P<0.01. Cul7, Cullin7; IC50, half maximal inhibitory concentration; LUAD, lung adenocarcinoma; qPCR, quantitative polymerase chain reaction; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymmethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt.